Merck, OP133-100UG, Anti-IKKα Mouse mAb (14A231)

General description
Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
Recognizes the ~85 kDa IKKα protein in Daudi cells.
This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.
Immunogen
Human
a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag sequence
Application

Immunoblotting (1-2 g/ml, chemiluminescence)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 0.2% gelatin, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
Daudi cells
Other Notes
Zandi, E., et al. 1998 Science281, 1360.
Didonato, J.A., et al. 1997 Nature388, 548.
Lee, F.S., et al. 1997 Cell88, 213.
Mercurio, F., et al. 1997 Science278, 860.
Regnier, C.H., et al. M. 1997 Cell90, 373.
Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA94, 11758.
Verma, I.M., et al. 1995 Genes. Dev.9, 2723.
Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)