Merck, OP17-200UG, Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and ~62 kDa v-fos proteins.
Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins. Does not cross-react with the ~39 kDa Jun protein.
This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).
Immunogen
Human
a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos
Application
Immunoblotting (2.5-5 µg/ml)
Immunocytochemistry (2.5-5 µg/ml)
Paraffin Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Analysis Note
Positive Control
HeLa cells
Other Notes
Jensen, D.E., et al. 1991. Oncogene6, 1219.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.
Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)