Merck, OP44-100UG, Anti-APC (Ab-1) Mouse mAb (FE9)

General description
Recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells.Antibody Target Gene Symbol: APC
Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
Entrez Gene Name: adenomatous polyposis coli
Hu Entrez ID: 324 (Related Antibodies: OP80, ST1150, OP62, OP47L)
Mu Entrez ID: 11789
Rat Entrez ID: 24205

Anti-APC (Ab-1), mouse monoclonal, clone FE9, recognizes full length APC (p300) in HCT116 cells and truncated APC (p147) in SW480 cells. It is validated for Western botting.
Protein G purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP40 cells. Recognizes the ~300 kDa APC protein as well as a variety of truncated forms.
Immunogen
a synthetic peptide corresponding to the N-terminal 35 amino acids of APC
Application
Immunoblotting (1 µg/ml, see comments)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, pH 7.5, 0.2% gelatin.
Analysis Note
Positive Control
HCT116 cells for p300, SW480 cells for truncated APC (p147)
Other Notes
Koetsier, P. A., et al. 1993. BioTechniques15, 258.
Smith, K. J., et al. 1993. Proc. Natl. Acad. Sci., USA90, 2846.
Su, L.-K., et al. 1993. Can. Res.53, 2728.
Boynton, R. F., et al. 1992. Proc. Natl. Acad. Sci. USA89, 3385.
D′Amico, D., et al. 1992. Cancer Res.52, 1996.
Fearon, E. R., and Jones, P. A., 1992. FASEB J.6, 2783.
Miyoshi, Y., et al. 1992. Proc. Natl. Acad. Sci. USA89, 4452.
Powell, S. M., et al. 1992. Nature359, 235.
Groden, J., et al. 1991. Cell66, 589.
Kinzler, K. W., et al. 1991. Science253, 661.
Nishisho, I., et al. 1991. Science253, 665.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)