Merck, P1004-10MU, Polymyxin B sulfate salt

General description
Polymyxin B sulfate is a strongly cationic cyclic polypeptide antibiotic that is derived from fermentation of Bacilus polymyxa. It is a mixture of B1 and B2 sulfate.
Application
Polymyxin B sulfate salt has been used:
in human aortic endothelial cells (HAEC) cell culture to exclude potential endotoxin contamination in recombinant adiponectin
in the analysis of the isolates
in endotoxin-neutralizing activity of α-amylase (AmyI-1)-18 (LAL assay)

Polymyxin B sulfate is a strongly cationic cyclic polypeptide antibiotic that is derived from fermentation of Bacilus polymyxa. It is a mixture of B1 and B2 sulfate. The product has been used clinically to treat infections of the urinary tract, meninges and blood stream caused by susceptible strains of Pseudomonas aeruginosa†. It also has uses studying multidrug-resistant pathogens††, as an immobilized agent for removal of endotoxins, and to induce pore formation in the membranes of cortex cells from excised sorghum roots.
Biochem/physiol Actions
Polymyxin B is one of the most important antibiotics used to treat gram-negative bacterial infections, including infections caused by carbapenem-resistant non-fermenters and carbapenem-resistant Enterobacteriaceae.
Mode of Action: Polymyxin B Sulfate binds to the lipid A portion of bacterial lipopolysaccharides†, disrupting the cytoplasmic membrane by inducing pores large enough to permit nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.

Antimicrobial spectrum: Has bactericidal action on most gram-negative bacilli††, including E. Coli and on most fungi and gram-positive bacteria.
Features and Benefits
High quality antibiotic suitable for mulitple research applications
Preparation Note
As supplied, the product should be stored at 2-8°C in the dark. No change was observed in retained samples after three years′ of storage in these conditions.
Polymyxin B sulfate is soluble in water at 50 mg/mL, producing a very slightly hazy, colorless to yellow solution. It has only minimal solubility in any organic solvent. For example, it has a solubility of 0.115 mg/mL in ethanol.

Stock solutions should be sterile filtered and stored at 2-8°C. They are stable at 37°C for 5 days.
Other Notes
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Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.