Merck, P1860-1ML, Protease Inhibitor Cocktail

General description
Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.
Application
Protease Inhibitor Cocktail has been used:
as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
in trypsin solution for trypsin-inhibited studies
as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.
Features and Benefits
Broad specificity
Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
Contains no metal chelators: Ensures compatibility with downstream applications
Convenient packaging: 1 mL in glass bottle
Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form
Solution in DMSO (D 2650, Hybri-Max).
Preparation Note
Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Other Notes
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Disclaimer
After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.