Description
General description
Picrylsulfonic acid solution is a hapten, which can bind to tissue proteins and turn into an antigen. This leads to several immunologic responses. Colitis induced by a 2,4,6-trinitrobenzene sulfonic acid (TNBS) is a type of delayed hypersensitivity reaction to haptenized proteins. Leukotriene B4 (LTB4) and the monhydroxyl fatty acids 5-hydroxyeicosatetraenoic acid (5-HETE), 12-HETE, and 15-HETE are the major inflammatory mediators involved in this TNBS-induced colitis. Ethanol when used in high concentration act as a vehicle for the TNBS administration.
Application
Picrylsulfonic acid solution has been used:
to assess the efficiency of carboxymethyl lysine (CML) modifications of exposed primary amines
to incubate zona intact bovine embryos for the differential staining of blastocysts
to induce irritable bowel syndrome (IBS) in mice and to study its effects on Clostridium butyricum
Hydrophilic modifying reagent. Derivatives with amino compounds can be regenerated with hydrazine.