Merck, P300-05, Porcine Coronary Artery Endothelial: PCAEC (Cryovial)

General description
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Arterial endothelial cells play critical roles in cardiac homeostasis, and insults to the endothelial cells have been linked to a variety of vascular diseases. Porcine artery endothelial cells provide a useful tool to study artery endothelial function and metabolism.PCAEC from Cell Applications, Inc. have been used to investigate a new mechanism of obesity-associated vascular disease by demonstrating that resistin, an adipocyte-derived cytokine implemented in insulin resistance and diabetes, can cause endothelial dysfunction in coronary arteries through oxidative stress and down-regulation of NO synthase (Kougias, 2005). Conversely, secretoneurin was shown to activate expression of NO synthase and calmodulin, leading to endothelial relaxation (Chan, 2011). PCAEC were also used in adhesion and transmigration experiments in a study investigating anti-inflammatory, cardioprotective effects of a novel adenosine-lidocaine drug formulation (Shi, 2012).
Application
cardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine production
Biochem/physiol Actions
Artery
Preparation Note
2nd passage, >500,000 cells in Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO
Can be cultured at least 12 doublings

Please refer to the PCAEC Culture Protocol.
Other Notes
Porcine Endothelial Cell Growth Medium that contains 10% FBS and 10% DMSO