Merck, P3563-10PAK, Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4

General description
Phosphate buffered saline with TWEEN® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.
Application
Phosphate buffered saline has been used in:

western blotting
enzyme-linked immunosorbent assay (ELISA)
telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
cell isolation

Packaging
Foil pouches
Preparation Note
Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.
Other Notes
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Legal Information
TWEEN is a registered trademark of Croda International PLC