Description
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
Packaging
Package size based on DEA units
Physical form
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Analysis Note
Protein determined by biuret.
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.