Merck, P5400-1KU, Pectinesterase from orange peel

General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Application
The enzyme from Sigma has been used as a standard during the measurement of pectin methylesterase activity in sea buckthorn juices. It has been used in the hydrolysis of cell wall pectins while studying phyllotaxis in Arabidopsis. It has also been used to catalyze pectin hydrolysis (using an ultrasonic measurement device) for monitoring biological processes.
Pectinesterase is used to hydrolyze methyl esters of pectin to pectate and methanol. It contains pectin methylesterase and pectin pectylhydrolase. Product P5400 is from orange peel and comes as a lyophilized powder. It contains (NH₄)₂SO₄ and sodium chloride. It has been used to study the pectin methylesterase gene.
Biochem/physiol Actions
Pectinesterase catalyzes the hydrolysis of the methyl esters of pectin to yield pectate and methanol.
Physical form
Contains (NH₄)₂SO₄ and sodium chloride
Analysis Note
Protein determined by biuret.
Other Notes
One unit will release 1.0 microequivalent of acid from pectin per min at pH 7.5 at 30 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer