General descriptionClick here to view the kit protocolGenElute 96 Well PCR Clean-Up Kit allows for high throughput purification of PCR products. The kit provides the necessary reagents for purification of highly pure PCR products. The DNA recovery is 75-90% for fragments of 100 bp to 10 kb and removes primers, primer-dimers, nucleotides, salts and polymerase.
Once the PCR reaction is complete, the reaction volume is adjusted and Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides, and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.
The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation, and microarray analysis.
ApplicationThe purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation and microarray analysis.
Biochem/physiol ActionsOnce the PCR reaction is complete, the reaction volume is adjusted and the Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.
Features and Benefits- Complete removal of primers and primer-dimers
- Innovative wash plate minimizes risk of cross-contamination
- Suitable for processing under vacuum and centrifugation
- Time-saving parallel clean-up of PCR products
Legal InformationGenElute is a trademark of Sigma-Aldrich Co. LLC
