Merck, PF0100-1KT, Protease Fluorescent Detection Kit

Application
Protease Fluorescent Detection Kit has been used:
in the detection of proteolytic activity using casein based protease assay
for testing the extracellular proteolytic activity of fungal strains
for quantifying mitochondrial protease activity
for measuring the protease production from soil solution

Fluorescent Protease Detection Kit Video Protocol
Detection limit: approx. 50 ng trypsin
Preparation Note
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.
Analysis Note
Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).