Merck, PF126-50UG, Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli

General description
Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Application
Neutralization (see biological activity)
Biochem/physiol Actions
EC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)