Merck, PP0200-1KT, GlycoProfile™ I, In-Gel Deglycosylation Kit

Application
The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained.
Biochem/physiol Actions
The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.
Preparation Note
Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC