Merck, PP0510-1KT, GlycoProfile™ IV Chemical Deglycosylation Kit

General description
The GlycoProfile™ IV Kit utilizes trifluoromethane sulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while safeguarding the protein or polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are eliminated. The conditions are optimized to deglycosylate simple or low molecular weight glycoproteins in 30 mins without the scavenger. For high molecular weight or complex, non-mammalian glycoproteins, anhydrous anisole is provided as a scavenger to give a high protein yield.
Application
GlycoProfile™ IV Chemical Deglycosylation Kit has been used in the deglycosylation of human α-L-iduronidase and peritrophic matrix from the gut of the caterpillar, Helicoverpa armigera. It has also been used to estimate the glycosylation of plant-derived recombinant human interleukin-13 (rhIL-13).
Features and Benefits
Eliminates both N- and O-linked glycans from proteins
This enables sequence analysis of the protein core by mass spectrometry (MS) or DNA-to-protein sequence comparison
Eliminates glycans which affect the molecular radius
Allows molecular mass determination by electrophoresis
Complete deglycosylation in as short as 30 mins for high throughput
Quick as it eliminates the need for multiple enzyme reactions
Accommodates a broad range of glycoproteins
Minimal degradation of the protein core to facilitate reliable MS results

Preparation Note
The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.
Legal Information
GlycoProfile is a trademark of Sigma-Aldrich Co. LLC