Merck, PPB002-20PAK, Tris Buffered Saline, with 0.05% Tween® 20, pH 8.0

General description
Tris Buffered Saline with 0.05% Tween® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.
Application
Suitable for use as a wash buffer in:
Western blot
ELISA
Immunohistochemistry

Features and Benefits
Ready-to-use buffer requires no measurments or pH adjustments
Saves time, money and effort to get your assays started fast
Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
Qualified for use in gel electrophoreis and other molecular biology assays

Packaging
Foil pouches
Preparation Note
Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
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Legal Information
TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC