Merck, PROTTWO-1KT, ProteoPrep® Universal Extraction Kit

Application
This kit features new and innovative detergents, and uses specially formulated reagents and an optimized protocol designed to generate two prepared subcellular fractions that are uniquely ready for two-dimensional (2D) electrophoresis.
Fraction 1: Soluble/Cytoplasmic Proteins
Fraction 2: Membrane Proteins
The special reducing and alkylating reagents produce samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Universal Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
Features and Benefits
Innovative detergent preparations - Highly improved solubility allows for higher protein loads and greater visibility of low abundance proteins on 2D gels.
Two pre-mixed solubilizaton solutions - Generates two distinct populations for easy 2D analysis.
Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
Pre-weighed dry blends - Stable and easy to reconstitute
Conveniently Packaged - No waste. Use only the amount needed.

Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany