General description
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic phosphotyrosine-containing peptide, followed by immunoaffinity chromatography. Recognizes the ~125 kDa FAK protein phosphorylated at Tyr⁸⁶¹.
Recognizes the ~125 kDa FAK protein phosphorylated at Tyr⁸⁶¹ in primary chick embryo fibroblasts transfected with human FAK.
This PhosphoDetect Anti-FAK (pTyr⁸⁶¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr⁸⁶¹).
Immunogen
a synthetic phosphopeptide surrounding the Tyr⁸⁶¹ phosphorylation site of human FAK
Physical form
In Dulbecco′s PBS (without Mg²⁺ and Ca²⁺), 50 % glycerol, 1 mg/ml BSA, pH 7.3 (±0.1).
Analysis Note
Positive Control
Chicken embryo fibroblasts (CEF) expressing FAK, plated on fibronectin
Other Notes
Eliceiri, B.P., et al. 2002. J. Cell. Biol.157, 149.
Hauck, C.R., et al. 2002. J. Biol. Chem.277, 12487.
Lacalle, R.A., et al. 2002. J. Cell. Biol.157, 277.
Lim, Y., et al. 2002. J. Biol. Chem.277, 12735.
Melendez, J., et al. 2002. J. Biol. Chem.227, 45203.
Rigacci, S., et al. 2002. J. Biol. Chem.277, 41631.
Datta, A., et al. 2001. Mol. Cell. Biol.21, 7295.
Nakamura, K., et al. 2001. Oncogene20, 2626.
Rocic, P., et al. 2001. J. Biol. Chem.276, 21902.
Slack, J.K., et al. 2001. Oncogene20, 1152.
Sieg, D.J., et al. 2000. Nature Cell. Biol.2, 249.
Vial, D., et al. 2000. J. Biol. Chem.275, 28269.
Wennerberg, K., et al 2000. Mol. Cell. Biol.20, 5758.
The immunogen sequence is 100% conserved in rat and Xenopus FAK but cross-reactivity has not been tested. This antibody is also reported to work for immunohistochemistry. Antibody should be titrated for optimal use in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)