Merck, PURD10005-1KT, Pur-A-Lyzer™ Midi Dialysis Kit

General description
Pur-A-Lyzer dialysis tube kits are easy-to-use devices in a screw-cap tube format and are disposable. They contain a dual dialysis membrane window for buffer exchange. Samples are added and removed using a standard laboratory pipette and come in 4 different versions to accommodate a range of sample volumes.

• High sample recovery >97%
• Protease-, RNase-, and DNase-free
• Convenient sample loading- no syringes necessary
• Versatile- dialyze proteins, RNA, and DNA

The Pur-A-Lyzer Dialysis Kits are an innovative dialysis tubing that can be conveniently loaded and unloaded using any standard pipette tip while still allowing for 98% sample recovery yield.

The revolutionary design of the Pur-A-Lyzer Tube makes it the only dialysis tool which enables true high throughput dialysis, accommodating a large number of samples in a single buffer tank.

The Pur-A-Lyzer Dialysis Tube allows rapid, high performance extraction of macromolecules without contamination. It is ideal for purification of proteins, automated protein sequencing, peptide mapping and amino acid analysis.
Application

• Dialysis or buffer exchange of volumes between 10 mL and 20 mL
• Preparation of protein samples for MALDI-MS
• Sample concentration
• Large-scale protein dialysis such as antibodies and recombinant protein purification
• Peptide dialysis for molecules as small as 10 amino acids
• Virus-particle purification
• Removal of contaminating micro molecules
• Purification of tissue culture extracts
• Removal of salts, surfactants, solvents and detergents
• Complex formation studies (protein-protein, protein-DNA and protein-RNA)
• pH and buffer adjustment of sample solution, protein extract or cell extract
• High through- put dialysis, a single beaker can be used for several Pur-A-Lyzer tubes on one floating pad

For your convenience the Pur-A-Lyzer kits come in four sizes:
Mini: 10-250 μL
Midi: 50-800 μL
Maxi: 0.1-3 mL
Mega: 3-20 mL
Legal Information
Pur-A-Lyzer is a trademark of Sigma-Aldrich Co. LLC