Merck, QPBCA-1KT, QuantiPro™ BCA Assay Kit

Application
The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.
In addition to protein determination and quantification in solution, the QuantiPro™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.
Biochem/physiol Actions
Based on the alkaline reduction of Cu²⁺ to Cu¹⁺ by proteins, and the formation of a bicinchoninic acid:Cu¹⁺ complex having an absorbance maximum at 562 nm.
Features and Benefits
Accurate across a broad range of protein concentrations
High sensitivity; linear response form 0.5 to 30 μg/ml of protein
Stable color complex
Reduced susceptibility to detergents

Preparation Note
Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.
Legal Information
QuantiPro is a trademark of Sigma-Aldrich Co. LLC