Merck, QR0200-1KT, Quantitative RT-PCR ReadyMix™

General description
Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.
Application
Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).
Features and Benefits
The master mix allows consistency and reproducibility from one reaction to the next
Reduced risk of contamination from multiple pipetting steps
Reduced set-up time as compared to manual or wax Hot Start methods
JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
Broad instrument compatibility
Includes a separate ROX reference dye vial for reaction normalization

Packaging
1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.
Legal Information
This product is for research use only.
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC