Description
General description
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Bone marrow is the major blood creating organ, but in addition to supporting hematopoietic growth and differentiation, marrow stromal cells can be induced to produce cells of other connective tissues, such as bone, cartilage, and fat, as well as cells from neuroectodermal (neurons) and endodermal (hepatocytes) lineages. The potential of HMSCs to maintain multipotency and proliferate extensively in vitro provides new avenues for cell-based therapy in the restoration of damaged or diseased tissue.RMSC from Cell Applications, Inc. have been used to demonstrate that TGF-β stimulates production of MCP-1 by vascular smooth muscle cells, which attracts bone marrow stromal cells (Zhang, 2009); and show that their migration can be stimulated by VPA and lithium through HDAC-CXCR4 and GSK-3β-MMP-9, respectively (Tsai, 2010); to investigate the therapeutic potential of marrow stromal stem cells depending on the extracellular matrix properties (Gershlak, 2013; Sullivan, 2014); and demonstrate that combination of low-level laser therapy and transplantation of marrow stromal stem cells results in greater functional recovery after nerve crush injury (Yang, 2013). Finally, HMSC were used to develop methods to direct stem cell differentiation by altering physical topography of the substrate (Brammer, 2011), and to design surface materials to control cell-adhesion properties (Sommani, 2010; Tsuji, 2011)
Application
Blood production, hematopoietic growth and differentiation, pathways of pluripotent cell differentiation into bone, cartilage and fat, connective tissue production,supportive structure, cell production, cell proliferation, cell based therapy, tissue restoration and repair, differentiation, effects of culture conditinos, HLA expression, cytokine production, immunomodulation, clinical trials.
Biochem/physiol Actions
Bone Marrow
Preparation Note
2nd passage, >500,000 cells in Rat Marrow Stromal Cell Basal Medium that contains 10% FBS and 10% DMSO
Can be cultured at least 10 population doublings
Please refer to the RMSC Culture Protocol.
Analysis Note
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Other Notes
Rat Marrow Stromal Cell Basal Medium that contains 10% FBS and 10% DMSO
