Merck, RNB700-10RXN, GenElute™Plasma/Serum RNA Purification Maxi Kit

General description
Click here to view the kit protocol
Sigma′s GenElute™ Plasma/Serum RNA Purification Maxi Kit provides a fast, reliable, reproducible and simple procedure for isolating circulating RNA and exosomal RNA from small plasma/serum inputs ranging from 2 mL and up to 5 mL, with various kit formats addressing different plasma/serum input volumes. Purification is based on spin column chromatography that uses Sigma′s proprietary resin separation matrix. The kit is designed to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA. Sigma′s GenElute™ Plasma/Serum RNA Purification Maxi Kit provides a clear advantage over other available kits in that they do not require phenol/chloroform or any protease treatments. RNA can be isolated from either fresh or frozen samples using these kits. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 10 μL to 100 μL. Typical yields of free-circulating and exosomal RNA vary depending on the input sample, as the amount of RNA present in plasma and serum will depend upon the health status of the individual. Normally, the RNA yield from plasma or serum RNA is highly variable (ranging from 1 to 100 ng/mL).

Isolate all sizes of circulating and exosomal RNA, including microRNA
Versatile plasma/serum input ranging from 2 mL to 5 mL
No phenol extractions
No carrier RNA
Concentrate circulating RNA and Exosomal RNA into a flexible elution volume ranging from 50 μL to 100 μL
Purify high-quality RNA in 35-40 minutes

Application
Reverse transcription qPCR
Reverse transcription PCR
Northern blotting
RNase protection and primer extension
Expression array assays
miRNA array
RNA-Seq
Nanostring
Fluidigm
Droplet/Digital PCR

Other Notes
Preps
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC