Merck, S1951-1UN, α-2,3-Sialyltransferase from Pasteurella multocida

Name: α-2,3-Sialyltransferase from Pasteurella multocida
Brand: Merck
SKU: S1951-1UN

Size:1 unit, CMP-N-acetylneuraminate:β-D-galactoside α-(2, 3)-N-acetylneuraminyltransferase, recombinant, expressed in E. coli BL21, ≥2 units/mg protein

Catalog Number: S1951-1UN

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Category

Recombinant Enzymes

Pack Size1 units

TagsProtein Biology Proteins & Enzymes Glycobiology Profiling Tools

CollectionsMerck Proteins & Enzymes: Nucleases, Albumins & High‑Purity Enzymes Merck Cell Culture Supplements & Reagents: Complete Solutions for Research & Bioproduction Merck Roche Life Science Products for PCR, RT‑PCR & Nucleic Acid Purification

Description

General description
Sialyltransferases belongs to the glycosyltransferases family with the capability of catalyzing the transfer of N-acetylneuraminic acid residues. It is a multifunctional enzyme with α-2,3-sialyltransferase activity, α-2,6-sialyltransferase activity, sialidase activity, and trans-sialidase activity. It has a molar mass of 46.4 kDa, with pI, pH being 5.94 and 7.5-8.5 respectively.
Biochem/physiol Actions
Sialyltransferase catalyzes the transfer of Neu5Ac from CMP-Neu5Ac as a donor substrate to the β-D-galactosyl-1,4-N-acetyl-D-glucosaminyl termini of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.
Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.
Physical form
Lyophilized powder containing Tris-HCl and NaCl
Preparation Note
Reconstitute the lyophilized powder with a volume of water in the range of 0.1 mL to 1 mL, to give a concentration in the range of 1 unit/mL (1 mL volume of water) to 10 units/mL (0.1 mL volume of water).
Analysis Note
Enzymatic activity assays performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5Ac (1 mM) and Lac-β−OMU (1 mM) at 37°C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm).
Other Notes
One unit will catalyze the formation of 1.0 μmol Neu-5-Ac-α-2,3LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0.

Specifications

General

UNSPSC Code	12352204
NACRES	NA.54
Enzyme Commission (EC) Number	2.4.99.4 ( BRENDA \| IUBMB )
MDL number	MFCD00130470
recombinant	expressed in E. coli BL21
form	lyophilized powder
specific activity	≥2 units/mg protein
mol wt	46.4 kDa
shipped in	dry ice
storage temp.	−20°C
Quality Level	200

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Merck, S1951-1UN, α-2,3-Sialyltransferase from Pasteurella multocida

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