Merck, S6639-1L, SSC Buffer 20× Concentrate

General description
SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.
Application
SSC Buffer 20× Concentrate has been used
as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon

Features and Benefits
Convenient ready-to-use liquid
Multi-purpose use in hybridizations

Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).