Description
General description
ApopTag Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3′-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag In Situ Apoptosis Detection Kits (Catalog #s: S7100, S7101, S7110, S7111, S7160 and S7165).
ApopTag is a registered trademark of Serologicals Company.
Application
Materials Provided:
Each vial contains 300 μl of ApopTag Terminal Transferase (TdT) Enzyme.
Instructions for Use
Prepare labeling mixture as follows:
77 μl ApopTag Reaction Buffer (S7105 or kit component # 02)
33 μl ApopTag TdT Enzyme
Mix well and keep on ice. This reagent may be prepared in advance and stored on ice for no more than 6 hours. The Reaction Buffer - TdT Enzyme mixture is ready for use at the "APPLY WORKING STRENGTH TdT ENZYME" step in The Complete ApopTag Manual.
Warnings and Precautions :
ApopTag Terminal Transferase (TdT) Enzyme contains potassium cacodylate (dimethylarsinic acid) as a buffer. This material is harmful if swallowed; avoid contact with skin and eyes (wear gloves, glasses); wash areas of contact immediately.
Packaging
1000 units/mL
Preparation Note
Recommended Storage -15°C to -25°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
