Description
General description
Alpaca Anti-Human IgG(Fcγ) Biotin single domain Antibody is designed to specifically detect human IgG Fcγ fragment. It is a monoclonal, single domain, recombinant antibody to human IgG Fc coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Human IgG(Fcγ) Biotin single domain Antibody reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG.
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Immunogen
Recombinant Alpaca single variable domain secondary nano antibody
Biochem/physiol Actions
Human IgG(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c
Packaging
100 μg in Glass bottle
Physical form
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
Preparation Note
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
Other Notes
Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
