General description
Product Source: Human ES Cells (Mel-1 cell line)
Application
Includes 1 million viable cells of Human Mesenchymal Stem Cells (derived from hES cells).
Mesenchymal stem cells (MSCs) are defined as a self-renewing population of adherent, multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined in vitro assays, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissue. Mesenchymal stem cells have been isolated from adult bone marrow, adipose tissue dermis, and other connective tissues. Isolation of MSCs from these sources typically requires invasive procedures and availability of a suitable donor. The number of MSCs that can be isolated from a single donor and the capacity for long term proliferation is limited. Mesenchymal stem cells derived from human embryonic stem cells provide an attractive alternative source to a potentially unlimited supply of cells with multilineage differentiation capacity.
EMD Millipore’s Human Mesenchymal Stem Cells (MSC) are derived from MEL-1 human embryonic stem cells (hESC). The cells proliferate as an adherent cell monolayer and can be expanded for up to 10 passages. Human ESC-derived MSC express the appropriate MSC markers including H-CAM (CD44), STRO-1, Thy-1 (CD90), and M-CAM (CD146) and do not express hematopoietic cell surface markers, CD14 (present on leukocytes) and CD19 (present on B-lymphocytes) and pluripotent markers, Oct-4 and SSEA-4. Cells can readily differentiate into adipocytes and osteoctyes.
EMD Millipore’s cryopreserved Human Mesenchymal Stem Cells derived from human ESC (Cat. No. SCC036) can be optimally expanded in low-serum media (FibroGRO LS Complete Media Kit for Culture of Human Fibroblasts, Cat. No. SCMF002). For serum-free applications, cells can also be expanded in serum-free media (FibroGRO Complete Media Kit for Culture of Human Fibroblasts, Cat. No. SCMF001).
Research Category
Stem Cell Research
Preparation Note
Human Mesenchymal Stem Cells(derived from hES cells) (Part # SCC036) should be stored in liquid nitrogen. It is recommended that the cells be used within ten passages.
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Analysis Note
EMD Millipore’s Human Mesenchymal Stem Cells are derived from MEL-1 human embryonic stem cells and have been validated for high expression level of cell surface molecules that are present on mesenchymal stem cells: H-CAM (CD44), M-CAM (CD146), THY-1 (CD90), and STRO-1 and for their absence of hematopoietic and pluripotent cell surface markers, CD14 (present on leukocytes), CD19 (present on B-lymphocytes) and Oct-4 and SSEA-4 (present on undifferentiated human ES cells), respectively. The cells have also been validated for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
