General descriptionEmbryonic Stem Cell line derived from C3H/HeN blastocysts. C3H/HeN is a widely used inbred strain, commonly used for pharmacological testing. F1 mice produced from C57BL/6 and C3H parental strains are commonly used in toxicological testing. C3H mice show a high frequency of mammary tumors when reared in non-SPF conditions due to viral transmission from mother to offspring during rearing. The C3H/HeN strain carries the rd1 (retinal degeneration) mutation. They carry the H2k haplotype. Additional phenotypic information is available at Mouse Genome Informatics website (http://www.informatics.jax.org/external/festing/mouse/docs/C3H.shtml). The C3H ES line was genome verified by determining the alleles at 29 SNPs that are informative across a large number of inbred mouse strains.
STRAIN: C3H/HeN
GENDER: Male
KARYOTYPE: 40XY
Packaging2.5 x 10⁶ cells per vial, passage 9
Physical formCells are supplied frozen in basal medium supplemented with 50% FCS and 10% DMSO
Preparation NotePlace vials in liquid nitrogen immediately upon receipt until it is convenient to proceed to subculture.
Other NotesSubject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerUnless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
