General descriptionThe AT-3 cell line was isolated from an autologous mammary tumor from cells originating in a transgenic MTAG mouse model . Mammary tumors arising in MTAG mice closely mimic changes observed in human breast carcinomas over the course of disease progression . AT-3 cells are a useful mouse model for studies of tumor physiology that are representative of typical disease states in humans.
Source:The AT-3 cell line was derived from primary mammary tumor cells of a transgenic MTAG mouse. The MTAG mouse model expresses the polyoma virus middle T antigen under control of the MMTV-LTR promoter .
Note: AT-3 cells demonstrate diverse morphologies in culture.
Tumor-induced immunosuppression is a common phenomenon in cancer and an important consideration for cancer immunotherapy. However, most studies on tumor-induced immunosuppression rely on tumor implant models, in which the disease progresses rapidly and usually causes non-physiological host responses that do not recapitulate normal tumor development and progression . Models that generate autochthonous tumors are therefore valuable for providing more physiologically-relevant systems for investigating tumor-host interactions and longer-term immunosuppressive effects.
ApplicationAT-3 mouse mammary tumor cell line may be used to develop mouse models for mammary cancer studies.
Research Category
Cancer
Oncology
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Biochem/physiol ActionsCancer Cells
Preparation NoteStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination
DisclaimerThis product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
