General descriptionThe GN11 cell line was derived from a transgenic mouse expressing Simian virus-40 (SV40) large T antigen driven by the human GnRH promoter (3). An olfactory bulb tumor from one male GnRH promoter-SV40T fusion-expressing transgenic mouse were dispersed and the GN11 cell line was established from serial dilution and cloning from the primary cells.
Gonadotropin-releasing hormone (GnRH) is important for control of reproduction in all mammals, acting to stimulate the synthesis and secretion of follicle-stimulating hormone and luteinizing hormone from the pituitary gland (1).GnRH is secreted by specialized neurons that migrate from the olfactory bulb to the brain during embryonic development. GnRH neurons are highly sensitive to glucose and insulin, suggestive of their physiological role in sensing the metabolic status in preparation for reproduction (2). Defects in GnRH neuronal function result in subfertility and developmental abnormalities. The scattered distribution and scarcity of GnRH neurons in the brain complicate the in vivo study of these cells and necessitate the utilization of robust in vitro cell models.
The GN11 mouse neuronal cell line is an immortalized cell line established as a model of GnRH-secreting neurons (3). In addition to GnRH expression, GN11 cells display neuron-specific markers including microtubule-associated protein 2 (MAP2) and Tau (4). GN11 cells express insulin receptor (5) and are responsive to steroid hormones (6). The GN11 GnRH-secreting neuronal cell line has proven a valuable system for exploring the cellular mechanisms of neuroendocrine reproductive physiology.
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Packaging≥1X10⁶ cells/vial
Preparation NoteStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis NoteEach vial contains ≥ 1X10⁶ viable cells.
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.
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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
