General descriptionAthymic nude mice xenografted with human carcinoma have been used as an in vivo model of tumor invasion and metastasis. Most reports indicate that metastasis of human epithelial tumors inoculated subcutaneously into nude mice is rather rare. HSC-3 is a human oral squamous carcinoma cell line that forms metastatic foci in the draining lymph nodes when subcutaneously transplanted into nude mice
. Conditioned media from HSC-3 has been shown to promote angiogenesis, HSC-3 cells having markedly elevated levels of growth factor secretion . The highly aggressive characteristics of this cell line have made HSC-3 a highly cited model for the study of metastatic squamous cell carcinoma.
Source:
The HSC-3 cell line was established from tumors of metastatic lymph nodes originated in tongue squamous cell carcinomas of a 63-year old male patient. Epithelial cells that migrated and proliferated were collected and subcultured from fresh explanted tumor tissues .
Squamous cell carcinoma is the most common malignant neoplasm found in the oral cavity and cervical metastases are frequently observed . Suppression of regional lymph node metastases is one of the most significant factors affecting favorable prognosis of squamous cell carcinoma . Experimental models of lymphogenous metastasis analogous to progression of human oral carcinoma are needed to provide an opportunity to investigate metastatic mechanisms.
Application
HSC-3 human tongue squamous carcinoma cell line is a suitable model for the study of metastatic squamous cell carcinoma.
Research Category
Cancer
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Biochem/physiol Actions
Cancer Cells
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Analysis Note
• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. 
