General descriptionThe growing worldwide obesity epidemic has stimulated research into novel strategies to control and treat obesity and associated metabolic disorders. Great interest has been generated in brown fat, a specialized adipose tissue having increased energy expenditure, because manipulation of this tissue has potential to protect against diet-induced obesity by altering the balance of energy intake and consumption (1). In common with brown fat, beige adipocytes possess thermogenic mechanisms, but have the unique property of inducible differentiation. Beige adipocyte differentiation occurs within white adipose tissue in response to environmental cues and is characterized by cristae-dense mitochondrial biogenesis and increased expression of uncoupling protein 1 (UCP1) (2).
The IngWAT mouse immortalized preadipocyte cell line is derived from subcutaneous white adipose tissue (WAT) isolated from a wild-type mouse (2). IngWAT cells have been shown to support the induction of beige adipocytes and express UCP1.2 IngWAT mouse immortalized preadipocyte cells are a versatile model for a broad spectrum of studies of beige adipocyte induction, maintenance and physiology.
References:
1. Cui XB, Chen SY (2016) White adipose tissue browning and obesity. 31(1): 1-2.
2. Lu X et al. (2018) Mitophagy controls beige adipocyte maintenance through a Parkin-dependent and UCP1-independent mechanism. Sci Signal 11(527): eaap8526.
ApplicationEach vial contains ≥ 1X10⁶ viable cells.
Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
Cells are negative for mycoplasma contamination.
Biochem/physiol ActionsAdipocytes
Preparation NoteStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other NotesSubject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerThis product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Clickhere for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
