General description
Hepatocellular carcinoma is the primary malignancy of the liver and a leading cause of cancer-related death in the world. The etiology of hepatocellular carcinoma is closely linked to infection with hepatitis B virus (1). Because of the causative role of HBV in liver cancers, cellular models of HBV-infected hepatocellular carcinomas have been developed for understanding the progression and fundamental biology of liver cancers.
The Hep-G2/2.2.15 human hepatoblastoma cell line, a subclone of HepG2 human hepatoblastoma that stably expresses the hepatitis B virus, is one of the most widely used models for HBV-associated liver disease. Hep-G2/2.2.15 cells possess lower proliferation and invasion activity than parental HepG2 cells and have low tumorigenicity in nude mice (2). Like parental HepG2 cells, Hep-G2/2.2.15 cells express the microtubule-associated marker doublecortin (DCX).
Source:
Hep-G2/2.2.15 was produced from Hep-G2 cells stably transformed with the hepatitis-B virus (HBV) genome. Hep-G2/2.2.15 cells are resistant to G418 and contain two head-to-tail dimers of the HBV type D genome (3). The parental HepG2 cell line was isolated from hepatoblastoma tissue of a 15-year-old male patient (4).
Research Category:
Cancer
Infectious Diseases
Biochem/physiol Actions
Human, Hepatocyte Cells;Cancer Cells
Packaging
≥1X106 cells/vial
Preparation Note
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
