General descriptionANBL-6 is a well-established model for MM.3 In culture ANBL-6 cell proliferation is dependent on IL-6, a critical component of the bone marrow microenvironment supporting tumor growth.2 Transcriptomic analysis of ANBL-6 cells demonstrated a high correlation with genetic signatures of primary MM tumors.2 ANBL-6 cells home to hematopoietic tissues in a mouse model, with a growth phenotype characteristic of smoldering (indolent) rather than an aggressive form of MM.2 Although derived from peripheral blood, ANBL-6 recapitulates many of the phenotypes associated with primary plasma cell tumors in the bone marrow. ANBL-6 is a highly representative cellular model of multiple myeloma.
ApplicationEach vial contains >1X10⁶ viable cells.
Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
Multiple myeloma (MM) is a cancer of the plasma cells originating in bone marrow. Although a rare cancer, there is no cure and only 54% of patients diagnosed with MM survive beyond 5 years.1 The vast majority of MM cases are known as smoldering or asymptomatic MM, wherein a small population of aberrant plasma cells resides in the bone marrow.2 Most MM cell line models are not derived from originating tissue but rather peripheral blood, raising questions of how closely these lines reflect the characteristics of the disease in the originating hematopoietic environment.2 SourceThe ANBL-6 cell line was isolated from peripheral blood of a 67-year old female patient with myeloma.3References1. Cancer Statistics: Multiple Myeloma https://www.cancer.net/cancer-types/multiple-myeloma/statistics Science 2004; 306(5694): 269-71.2. Leukemia 2020; 34(10): 2754-2765.3. Cancer Res 1993; 53(21): 5320-5327.
Features and BenefitsANBL-6 is a highly representative cellular model of multiple myeloma.
Preparation NoteANBL-6 Human Plasma Cell Myeloma Cell Line should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other NotesSubject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
DisclaimerRESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
