General description
Small cell cancer of the ovary hypercalcemic type (SCCOHT) is a rare form of highly aggressive cancer that primarily affects young women between the ages of 13 to 35. Very few cell lines or other models are currently available to study this devastating form of cancer. Although this cancer is often diagnosed at early stages, it has an especially poor prognosis with more than half of patients diagnosed with stage IA dying within two years. This type of cancer has been distinguished from other related malignancies including transitional cell carcinoma of the ovary, ovarian epithelial tumors, and ovarian germ cell tumors. Many other cell lines are available to study different ovarian carcinoma types, but no cellular model appears to exhibit the unique properties of SCCOHT-1. Biopsy material from a recurrent SCCOHT tumor was used for explant culturing. The new culture that became adherent and continued to proliferate was labeled “SCCOHT-1.” Characterization of the SCCOHT-1 cell line reveals an average diameter of 13 um and an estimated 36 hour doubling time. SCCOHT-1 cells are positive for CD29 and negative for CD24 and CD45 as assessed by flow cytometry. Injection of distinct subpopulations of the SCCOHT-1 cell line exhibited tumor development 100% of the time in NOD/SCID mice.1 Additionally, research groups have identified mutations resulting in loss of SMARCA4 protein which appears to be highly specific for SCCOHTs. SMARCA4 protein forms an important ATPase of the SWI/SNF chromatin remodeling complex which is also often implicated as a tumor suppressor. SCCOHT-1 cells provide a new tool to study the rare SCCOHT form of cancer and for the potential development of relevant therapeutic strategies in the future.Source:SCCOHT-1 cell line was created through explant culturing of a biopsied recurrent SCCOHT tumor taken from a 31-year old female patient.References: 1. Otte A, Göhring G, Steinemann D, Schlegelberger B, Groos S, Länger F, Kreipe H-H, Schambach A, Neumann T, Hillemanns P, et al. 2012. A tumor-derived population (SCCOHT-1) as cellular model for a small cell ovarian carcinoma of the hypercalcemic type. Int J Oncol. 41(2): 765-775.2. Karnezis AN, Wang Y, Ramos P, Hendricks WP, Oliva E, D′Angelo E, Prat J, Nucci MR, Nielsen TO, Chow C. 2016. Dual loss of the SWI/SNF complex ATPases SMARCA4/BRG1 and SMARCA2/BRM is highly sensitive and specific for small cell carcinoma of the ovary, hypercalcaemic type. J Pathol. 238(3): 389-400.
Application
Each vial contains >= 1X106 viable cells.
SCCOHT-1 cells are verified to be of human origin and negative for mouse, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells tested negative for infectious diseases against a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells tested negative for mycoplasma.
Features and Benefits
The SCCOHT-1 human ovarian SCC cell line derived from a 31yo female patient is a unique model for the study of small cell carcinoma of the ovary.
Preparation Note
SCCOHT-1 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
