General description
The RET oncogene was originally created by an accidental in-vivo recombination of two human genes, Ret and RFP (the Ret-finger protein then, known today as TRIM27), during a transformation of the NIH3T3 cells with human lymphoma DNA.1 The founder Ret tran
The mouse brain-tropic RET melanoma-sorted (BT-RMS) cell line is a derivative of the Ret-melanoma sorted cell line (RMS, Cat. No. SCC462). The RMS parental cells were isolated from a spontaneously occurring skin tumor in RET transgenic mice. SCC465 cells were derived from brain tumors formed after intracardiac injection of Ret malanoma-sorted (RMS) cells.
Application
Each vial contains >1X106 viable cells.
BT-RMS cells are verified to be of mouse origin and negative for human, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells tested negative for infectious diseases against a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
Cells tested negative for mycoplasma.
Features and Benefits
SCC465, the BT-RMS brain-tropic RET melanoma-sorted mouse cell line, expresses mCherry and exhibits increased incidence and shortened timeline of brain metastasis post-subdermal injection.
Preparation Note
BT-RMS cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
