Application
Cell Culture Cell Function Assay In vitro Bioassay in vitro Toxicology Assays Lung disease control
HBEC30KT bronchial epithelial cell line is useful both as normal control for NSCLC cell lines and for knockdown/knockout modifications for investigating tumor progression.
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Preparation Note
HBEC30KTcells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
Other Notes
Each vial contains greater than 1X106 viable cells.
Cells are tested negative for infectious diseases by a Contamination CLEAR Panel by Charles River Animal Diagnostic Services.
Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
Bronchial epithelial cells line the airways and provide critical functions in protection from pathogens and inhaled particulates. Damage to airway epithelial cells by carcinogens such as those present in tobacco smoke is a key etiological agent of lung cancer. Non-small cell lung cancer (NSCLC) comprises 85% of lung cancers, and includes both subsets, lung adenocarcinoma and lung squamous cell carcinoma. NSCLC arises from epithelial cells in the lung, mutations in epithelial growth factor receptor (EGFR) or Kras being the most common initiators. Mutations in tumor suppressor genes TP53 and CDKN2A drive tumor progression; respectively, these genes are inactivated in 90% and 70% of non small cell lung cancers. Although surgical resection is standard treatment in early-stage lung cancer, recurrence is common and lung cancer remains the number 1 cause of cancer deaths worldwide.
The HBEC30KT human bronchial epithelial cell line is a normal bronchial epithelial cell line immortalized with hTERT. HBEC30KT is useful both as normal control for non small cell lung cancer cell lines and for knockdown/knockout modifications for investigating genetic and molecular players in tumor progression. HBEC30KT expresses normal epithelial cell markers including beta-catenin.
Source
The HBEC30KT cell line was derived from normal human bronchial epithelial cells immortalized with hTERT.
References
1. Herbst RS et al. 2018. The biology and management of non-small cell lung cancer Nature 553:446-454.
2. Zaganjor E et al. 2014. Ras regulates kinesin 13 family members to control cell migration pathways in transformed human bronchial epithelial cells. Oncogene 33:5457-5466.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled.
