General description
≥1x106 viable HCC95 Lung Squamous Carcinoma Cancer Cells: (Catalog No. SCC483). Store in liquid nitrogen.
Application
Each vial contains >_ 1X106 viable cells.
Cells are tested negative for infectious diseases by a Human Essential CLEAR Panel by Charles River Animal Diagnostic Services.
Cells are verified to be of human origin and negative for interspecies contamination from mouse, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
Cells are negative for mycoplasma contamination.
Lung cancer is the leading cause of cancer death, causing 23% of all cancer mortality in 2020. Among all lung cancer cases, 85% are categorized as non-small cell lung cancer (NSCLC) which is a heterogeneous group of diseases with poor prognosis and a 5-year survival rate of less than 20%. Among all NSCLC cases, 30-40% are categorized as lung squamous carcinoma (LUSC) which often affects the main airways and bronchi and is attributed to smoking as its principal etiology.
The HCC95 cell line is a human lung squamous cell carcinoma line, collected from a metastatic site of pleural effusion. Recent transcriptomic characterizations show that HCC95 show high similarity to the typical LUSC transcriptome.
Source
HCC95 human squamous lung carcinoma cells were derived from a 65-year-old male with NSCLC.
References
1.J Natl Compr Canc Netw. 2013, 11(6): 645–653 DOI: 10.6004/jnccn.2013.0084
2.Nat Rev Cancer 2014, 14(8): 535–546 DOI: 10.1038/nrc3775
3.J Thorac Dis. 2013, 5(Suppl 5): S463–S478 DOI: 10.3978/j.issn.2072-1439.2013.08.43
4.J Clin Oncol. 2010, 28(36): 5311–5320 DOI: 10.1200/JCO.2010.28.8126
5.Clin Can Res. 1999, 5(5): 991-1000
6.Nat Commun. 2019, 10(1): 3574 DOI: 10.1038/s41467-019-11415-2
7.J Cancer Res Clin Oncol 2010, 136(4): 493–502 DOI: 10.1007/s00432-009-0681-6
Features and Benefits
Among the NSCLC cell lines, HCC95 has been shown to retain the lung squamous carcinoma (LUSC) disease phenotype. Early research showed that HCC95 is very similar to the original tumor genetically and histochemically, with poor differentiation and formation of keratinization. Collectively, these demonstrate that HCC95 is a valuable cell line for modeling LUSC with high fidelity in vitro. A 2010 study has exemplified its use in studying NSCLC sensitivity to cisplatin treatment.
Preparation Note
HCC95 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
Other Notes
Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact
[email protected].
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
