General descriptionCF™488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection conditions, CF™488A is at least as bright as Alexa Fluor™ 488. However, a major advantage of CF™488A over Alexa Fluor™ 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor™ 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF™488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF™488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor™ 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF™488A offers the advantage of less fluorescence "spill-over" in the red channel in multi-color detection applications.
CF™ dye hydrazides can be used as a fixable polar tracers for visualizing cell morphology,or for gap junction studies. The dyes can be introduced into cells by microinjection. For conjugation to aldehyde or ketone groups (such as carbohydrate molecules after peroxidation with periodate) we recommend using the more reactive CF aminooxy dye forms.
Features and Benefits1. Yields biologically more specific antibody conjugates and has less "spill-over" fluorescence in the red channel than Alexa Fluor™488. 2. Extremely photostable. 3. Highly water-soluble and pH-insensitive.
Preparation NoteDissolve in water
Protect from light
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[email protected].
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.
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