Description
General description
PluriSTEM-XF Human ES/iPS Medium is a complete xeno-free medium formulation for the feeder-free culture of human ES and iPS cells. Pluripotent cells maintained in thissmall molecule based medium exhibit high cell health, express high levels of pluripotency markers (NANOG, OCT3/4, SOX-2, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81), retain differentiation potential and possess a normal karyotype. PluriSTEM-XF Human ES/iPS Medium is provided as a stand-alone 500 mL bottle that is ready-to-use and does not require additional supplementation.
For a complete xeno-free culture system, PluriSTEM-XF Human ES/iPS Medium should be used with PluriSTEM-XF Recombinant Vitronectin (Cat. No. CC130).
Each lot of PluriSTEM-XF Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES/iPS cells for 3 passages.
Note: Before starting PluriSTEM-XF, users are recommended to read the manual carefully and completely. Colony morphology and growth rates may differ from other serum-free, feeder-free media culture conditions.
Application
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Preparation Note
PluriSTEM-XF Human ES/iPS Medium is provided frozen and can be stored at -20°C until the expiration date on the product label.
Before use, thaw PluriSTEM-XF medium at room temperature (15 – 25°C) or overnight at 2 – 8°C. Do not thaw at 37°C. Upon thawing, PluriSTEM-XF medium may be aseptically dispensed into working aliquots and stored at -20°C until the expiration date as indicated on the label. Thawed aliquots may be stored at 2 – 8°C for up to 2 weeks. Do not refreeze aliquots after thawing. Before use, warm working aliquots to room temperature. Do not warm the medium in a 37°C water bath.
Analysis Note
Each lot of PluriSTEM® Human ES/iPS Medium is rigorously quality control tested for the ability to sustain undifferentiated human ES cells for greater than 3 passages.
Legal Information
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
