Description
General description
Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic. Accutase is direct replacement for trypsin cell detachment solution and does not require enzyme inactivation with serum. Accutase does not contain mammalian or bacterial derived products.
AccutaseXL is a lyophilized form of Accutase with extended stability. AccutaseXL, once reconstituted, will make 500ml of complete 1X Accutase solution. Note: read detailed reconstitution protocol found in the product datasheet before using this product.
Product Source: Accutase is derived from a non-mammalian (invertebrate) source.
Application
Accutase has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Physical form
1X Accutase enzymes in Dulbecco’s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA4Na and 3 mg/L Phenol Red.
Preparation Note
Store lyophilized AccutaseXL at -20°C for up to 4 months from date of receipt.
Once reconstituted in water, liquid Accutase can be aliquoted and frozen at -20°C for 2 years or stored for 2 months at 4°C. It is recommended to thaw liquid Accutase at 4°C overnight or in a bath of cool water.
Analysis Note
pH: 6.8-7.8
Activity Assay: 500-20 units/ml (1 unit will release 1 µmole pNA from SUCR-pNA per minute at 37˚C)
Functional Assay: Cell detachment assay
Legal Information
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
