Description
General description
EMD Millipore′s Human iPSC Derived Neural Progenitor Kit contains human neural progenitor cells (NPCs) along with optimized medium for their expansion. Human neural progenitor cells (NPCs) are derived from induced pluripotent stem (iPS) cells generated using STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) lentivirus. The viral transgenes have been removed using cell permeable TAT-Cre recombinase (Cat. No. SCR508) with excision confirmed by quantitative PCR of genomic DNA extracted from human iPSC cells.
These hiPSC-derived NPCs proliferate as an adherent cell monolayer with greater than 80% expression of the appropriate neural stem cell markers, including Nestin and Sox-2. Cells are provided at passage 3 and can be expanded for a further three to five passages with ENStem-A Neural Expansion Medium (Cat. No. SCM004). Human NPCs can be further differentiated to terminal neurons by the use of the Human ES/iPS Neuronal Differentiation Medium (Cat. No. SCM111) or ENStem-A Neuronal Differentiation Medium (Cat. No. SCM017). Differentiation leads to greater than 80-90% of the cells expressing mostly neuronal markers, bIII-tubulin and MAP2.
Human iPSC-derived NPCs can be used for a variety of research applications including studies of neurotoxicity, co-culture applications and screening for molecules that induce or inhibit preferential differentiation to mature neurons.
Preparation Note
ENStem-ATM Neural Expansion Medium (Cat. No. SCM004) should be stored at -20C upon receiving.Human iPSC-Derived Neural Progenitors Cells (Cat. No. SCC035) should be stored in liquid nitrogen immediately after receiving. Temperature fluctuations will impair the recovery of healthy cells. Do not thaw the human cells until you are ready to do the assay of choice.
Other Notes
1) 1x10⁶ viable Human iPSC Derived Neural Progenitors: (Catalog No. SCC035). Store in liquid nitrogen. 2) 500 mL ENStem-A Neural Expansion Medium (Catalog No. SCM004). Store at -20c.
Disclaimer
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
