Description
General description
Absorbance Maximum: 510nm
Emisson Maximum: 670nm
Suggested Filters:
Excitation: 458/488/543nm
Emisson: 670-800nm
Long-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days in vitro and 21 days in vivo. These properties make them optimal candidates for long interval live cell bioimaging experiments.
The Luminicell Tracker-670 Cell Labeling Kit contain red fluorescent AIEDot nanoparticles with TAT sequences. These nanoparticles can be used to fluorescently tag cells for long term cell tracking and tracing experiments.
Features and Benefits
● Brighter: 10X brighter than other cell labeling technologies
● Photostable: 3X longer fluorescence without signal quenching
● Biocompatible: Non-toxic organic nanoparticles intended for biological applications
● Rapid Protocol: Easy-to-use protocol labels cells within 4 hours
Physical form
200nM in 0.5ml PBS, pH 7.4
Preparation Note
Store at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.
Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker three times for 1 min each before use.
Analysis Note
Absorbance: 510+/-5nm
Concentration: 180-220nM
Fluorescence: 665+/- 10nm
Brightness at 670nm: ≥1.7X10^7 M-1cm-1
Cellular Assay: HeLa Cell Fluorescence
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
