Description
General description
Singlet oxygen (1O2) is one of the reactive oxygen species (ROS). Singlet oxygen is known to be a cause of spots and wrinkles of the skin due to its very strong oxidizing potential. However, the reactivity of singlet oxygen, if controlled, can be used for therapy. In the field of cancer research and therapy, singlet oxygen is being used for photodynamic therapy (PDT), an emerging anticancer treatment using photoirradiation and photosensitizers.
BioTracker Si-DMA Singlet Oxygen Live Cell Dye is a novel far-red fluorescence probe that is composed of silicon-containing rhodamine and anthracene moieties as a chromophore and a singlet oxygen reactive site, respectively. In the presence of singlet oxygen, fluorescence of BioTracker Si-DMA live cell dye increases 17 times due to endoperoxide formation at the anthracene moiety. The dye is highly selective to singlet oxygen, among seven different reactive oxygen species (1O2, O2-, H2O2, HOCL-, RCO-, OH-, NO). Among three different intracellular photosensitizers, BioTracker Si-DMA selectively detects the 1O2 that is generated by 5-aminolevulinic acid-derived protoporphyrin IX, localized in mitochondria. On the other hand, mitochondria-targeted KillerRed and lysosomal porphyrins could not induce fluorescence change in the dye.
Spectral Properties
Absorbance: 640 nm
Emission: 670 nm
Application
Live cell fluorescent imaging
Live cell imaging dye for singlet oxygen (¹O₂) localized to mitochondria. Singlet oxygen is important in the field of cancer therapy, photodynamic medicine and other skin treatments.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye
Physical form
Lyophilized
Preparation Note
Store BioTracker Si-DMA Singlet Oxygen Live Cell Dye at 0-5°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 513.77 g/mol
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
