Description
General description
Cyclooxygenase (COX), also known as Prostaglandin-endoperoxide synthase (PTGS), is the key enzyme in prostaglandin biosynthesis. There are two isozymes of cyclooxygenase: a constitutive COX-1 and an inducible COX-2, which differ in their regulation of expression and tissue distribution. COX-2 overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX-2 activity within its native environment poses an exciting approach to investigate the effect of the local environments on protein activity.
BioTracker CoxFluor Live Cell Dye is an isoform selective activity-based sensor for COX-2. The probe may be used for COX-2 inhibitor screening assays and for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX-2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen dependent changes in COX-2 activity that are independent of protein expression within live macrophage cells.
Spectral Properties
Absorbance: 540 nm
Emission: 590 nm
Reference:
1. Yadav, A. K., Reinhardt, C. J., Arango, A. S., Huff, H. C., Dong, L., Malkowski, M. G., ... & Chan, J. (2020). An Activity?Based Sensing Approach for the Detection of Cyclooxygenase?2 in Live Cells. Angewandte Chemie, 132(8), 3333-3340.
Application
Purity ≥ 95% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.
Features and Benefits
Fluorescent probe for monitoring COX-2 activity in live cells by confocal microscopy or flow cytometry.
Physical form
Lyophilized. Off-white solid.
Preparation Note
Store at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
Other Notes
Live cell fluorescent imaging
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
