Description
General description
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker NIR694 Nuclear Dye (DMSO) is a cell membrane-impermeable, far-red dye with high selectivity for membrane compromised or dead cells. Dye staining is nuclear specific in fixed and permeabilized cells and tissue sections and does not require RNase treatment. The dye can be excited by wavelengths from 488 to 647nm and emits far red fluorescence with emission maximum at 694nm. For live cell nuclear staining we recommend the BioTracker NIR694 Nuclear Dye (Water).
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Application
Far red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. The BioTracker NIR694 Nuclear Dye (DMSO) is supplied as a 200X stock solution in DMSO.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye
Physical form
Liquid
Preparation Note
Store BioTracker NIR694 Nuclear Dye (DMSO) at 2-8C. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Spectral Properties
Absorbance: 662nm
Emission: 694nm
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
