Description
General description
The nucleus is a membrane-enclosed organelle found in eukaryotic cells. Cell nuclei contain most of the cell′s genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. Traditionally, DNA stains such as DAPI and Hoechst have been used for microscopy but require fixation and cannot be used for live cell imaging.
The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low toxicity for live cell imaging. The dye is supplied with a vial of verapamil, an efflux pump inhibitor that may improve probe retention and live cell staining in certain cell types.
Note: BioTracker Nuclear Dyes also shows blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.
Spectral Properties
Absorbance: 500nm
Emission: 515nm
Application
Live cell fluorescent imaging
Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye
Physical form
Liquid
Preparation Note
Store BioTracker 488 Green Nuclear Dye at 2-8ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Spectral Properties
Absorbance: 500nm
Emission: 515nm
Other Notes
1) 1 vial of 50µL BioTracker 488 Green Nuclear Dye (1000X in DMSO) (CS224594)
2) 1 vial of 100µL Verapamil HCL (100mM in DMSO) (CS224592)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
