Description
General description
Mitochondria are organelles found in large numbers in most cells, in which the biochemical processes of respiration and energy production occurs. In metabolically active cells, mitochondria produce a membrane potential by maintaining a proton gradient across the inner and outer membranes. Loss of mitochondrial membrane potential is a hallmark for apoptosis and poor cell health.
BioTracker Mitochondria dyes are fluorogenic stains for staining mitochondria in live cells. The dyes are membrane permeable and become brightly fluorescent upon accumulation in the mitochondrial membrane. Staining depends on mitochondrial mass, and not on mitochondrial membrane potential. Signal is lost as mitochondrial membrane integrity is lost during cell death.
The BioTracker 488 Green Mitochondria Dye can be detected using the green FITC/GFP channel. Also, the green staining is not dependent on membrane potential, and can be used to stain both live and fixed cells.
Spectral Properties
Absorbance: 490nm
Emission: 523nm
Application
Live cell fluorescent imaging
Live cell imaging mitochondrial dye that stains the membrane of mitochondria used to detect cell viability, metabolic activity and overall cell health.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye
Physical form
Lyophilized
Preparation Note
Store BioTracker 488 Green Mitochondria Dye at -20ºC. Protect From Light.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Analysis Note
Spectral Properties
Absorbance: 490nm
Emission: 523nm
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
