Description
General description
Exosomes are a form of extracellular vesicle (EV), which are known to contribute to intercellular communication. To investigate such interactions, labeling techniques based on fluorescent dyes have been employed. Fluorescent dyes that label the plasma membrane are commonly used for exosome labeling because the lipid bilayer in exosomes is a suitable labeling target.
The BioTracker™ exosome membrane labeling probe series can be used for staining the membranes of purified exosomes, which in turn permits imaging of labeled exosomes taken up by cells.SCT267 does not cause aggregation and has little impact on the biological features of exosomes, permitting accurate observation of exosome dynamics.BioTracker™ exosome membrane labeling probes vanquish issues with existing labeling dyes, including:1) self-particle formation2) an increase in size of the exosomes after labelingThree different fluorescence colors---green (SCT265), red (SCT266), and far red (SCT267)--- enable application in multiplexing experiments, such as observation of exosome localization in organelles.Add 10 uL DMSO to tube of green, red, or far red exosome membrane dye to create stock solution.SPECTRAL PROPERTIES:Ex 640nm/Em 640-760nmReferences:Kawata R, Oda S, Koya Y, Kajiyama H, Yokoi T. 2020. Macrophage-derived extracellular vesicles regulate concanavalin A-induced hepatitis by suppressing macrophage cytokine production. Toxicology. 443:152544.
Features and Benefits
This live cell probe fluoresces in the far red spectral range when it binds to the lipid bilayer in exosome membranes.
Preparation Note
Store BioTracker™ Far Red Exosome Membrane Labeling Kit at 2 - 8 °C, protected from light.After resuspending in DMSO, store stock solution at -20 °C, protected from light.
Other Notes
This live culture probe for the detection of exosomes labels the lipid bilayers of exosomal membranes, enabling observation of the uptake of exosomes by cells. Exosomes should be purified before labeling.
Legal Information
BioTracker is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
